T6557
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 | Immunofluorescence COS cells undergoing mitosis transfected with doublecortin-GFP (DCX) (green) were stained using Monoclonal Anti-γ-Tubulin, clone no. GTU-88 (Cat. No. T6557) with Anti-Mouse IgG-TRITC (Cat. No. T6258) (red) and DAPI (DNA, blue). From Robert McNeil, Pediatrics Department, Baylor College of Medicine, Houston, TX. |
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T6557
Sigma
Monoclonal Anti-γ-Tubulin antibody produced in mouse
clone GTU-88, ascites fluid
| MDL number: | MFCD00677366 |
Description
| Immunogen | synthetic γ-tubulin peptide (amino acids 38-53), conjugated to KLH. |
| Specificity | The antibody recognizes an epitope located within the N-terminal region of γ-tubulin. |
Properties
| antibody form | ascites fluid |
| clone | GTU-88 (monoclonal) |
| mol wt | antigen mol wt 48 kDa |
| contains | 15 mM sodium azide |
| species reactivity | Xenopus, canine, chicken, hamster, human, rat, bovine, mouse |
| application(s) | immunoblotting: 1:10,000 using cultured chicken fibroblast extract |
| immunocytochemistry: 1:5,000-1:10,000 using HeLa cells | |
| indirect ELISA: suitable | |
| isotype | IgG1 |
| Gene Information | human ... TUBG1(7283) |
| rat ... Tubg1(252921) | |
| mouse ... Tubg1(103733) | |
| shipped in | dry ice |
| storage temp. | −20°C |
Safety
| WGK Germany | 3 |
References
| reference | Sutton, A.L., et al., Ovarian Hyperstimulation Induces Centrosome Amplification and Aneuploid Mammary Tumors Independently of Alterations in p53 in a Transgenic Mouse Model of Breast Cancer Oncogene 3, e2366, (2008) |
Carpenter, A., et al., Mutations in Drosophila Greatwall/Scant Reveal Its Roles in Mitosis and Meiosis and Interdependence with Polo Kinase PLoS Genet. 3, e200, (2007) Abstract | |
| Zhang, L., et al., Mutual Inhibition between Kaposi’s Sarcoma-Associated Herpesvirus and Epstein-Barr Virus Lytic Replication Initiators in Dually-Infected Primary Effusion Lymphoma PLoS ONE 3, e1569, (2008) Abstract |
